Nucleotides Reveal Polynucleotide Phosphorylase Activity from Conventionally Purified GroEL
نویسندگان
چکیده
منابع مشابه
From polynucleotide phosphorylase to neurobiology.
In the fall of 1944, I enrolled at the Hebrew University of Jerusalem. The student body numbered about 700 and the choice of faculties was somewhat limited.My hopewas to study medicine, but the plans to open a medical school were still at the drawing board stage. I therefore chose to study chemistry with biochemistry and bacteriology as minor subjects, which I thought I would need later if I we...
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The enzyme, polynucleotide phosphorylase, catalyzes the polymerization of nucleoside diphosphates to polyribonucleotides with the formation of inorganic orthophosphate (1, 2). The reaction is readily reversible, and the phosphorolysis of polyribonucleotides has been studied extensively (3-6). Several recent reviews (7-9) afford extensive summaries of the literature. In the accompanying paper (l...
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A method is described for the preparation of p-aminophenyl oligo(dT)-Sepharose. This matrix has been used for the purification of polynucleotide phosphorylase from both E.coli and B.stearothermophilus. The effects of temperature and pH on the binding of the different enzymes to the matrix have been investigated. B.stearothermophilus isolated by affinity chromatography may be useful in selective...
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1. A new method has been developed for the preparation in good yield of highly purified Azotobacter vinelandii polynucleotide phosphorylase in its reduced form. 2. Aging or digestion with trypsin causes the enzyme to develop a primer requirement that is not eliminated by beta-mercaptoethanol. 3. The development of a primer requirement is accompanied by marked changes of the electrophoretic mobi...
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A simple procedure for purifying polynucleotide phosphorylase from Escherichin coli cells by means of affinity chromatography on an RNA-Sepharose column is described. The purified enzyme preparation has a specific activity 3500-fold that of the crude extract and is essentially homogeneous, as determined by ultracentrifugation, polyacrylamide gel electrophoresis under denaturing conditions, isoe...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.41.25063